He conducted cancer research on the bioenergetics of cell division under a grant from the Cancer Institute of the National Institutes of Health.  He holds ten patents, including functional raw materials, bovine teat dip and anticellulite hosiery.
Dr. Pugliese is a member of the Society of Bioengineering and the Skin, the American Academy of Dermatology, the Society of Investigative Dermatology and has given more than 125 presentations at scientific meetings worldwide.  He was awarded the Maison de Navarre Gold Medal, the highest honor of the National Society of Cosmetic Chemists for his contributions to the field of cosmetic chemistry.  Dr. Pugliese has contributed chapters to over a dozen books and published over 60 scientific papers. He has authored four books, including, in 1990, the first textbook written for estheticians by a physician, Advanced Professional Skin Care, to bridge esthetics and medicine with a common language.  With the publication of this Medical Edition, Dr. Pugliese continues to strive toward a referral-based skin care industry by educating practitioners at all levels.

Dr. Pugliese founded the Circadia Skin Care Institute in 2003, where he teaches advanced courses for the skin care professional in skin physiology, histology, biochemistry, cosmetic chemistry and medical esthetics.  His goal is to elevate the practice of esthetics to the status of a profession through higher standards of education in the United States.

Dr. Pugliese and his wife, Joanne, married since 1949, have four children, four grandchildren, and reside in Bernville, Pennsylvania.